Cyanobacterial strains that did not lyse until the 12th day were defined as insusceptible. Wavelength Range. EC migration was studied by wound healing assay. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Product Overview VersaMax™ ELISA Microplate Reader Manufacturer Molecular Devices® | Available Worldwide Be the first to review this product Request Pricing Molecular Devices® Manufacturer Website. 온도 균일성 ±0. As a result, assay flexibility is consequence-free. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . Never order or replace filters to optimize an assay again! Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. 0. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Wavelength Accuracy. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). 0 (Molecular Devices, Sunnyvale, CA). It also integrates with the. BMG Labtech - THERMOstar. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. Then the medium was removed and 200 μL of DMSO was added to each well to solubilize the formazan crystals. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Intracellular triglyceride assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. Supplier: Molecular Devices. Experimental data analysis was performed with a one-way analysis of variance (Anova with Newman-Keuls post-test) in Prism 6. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Bovine serum albumin was used as a reference. The final mixtures were allowed to react at 25 °C in the dark for 5 min. printed in u. View the supported Window operating systems for the different versions of the SoftMax Pro software. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum. 00 USD. Assays were conducted in a 96-well microplate using ELISA microplate reader (VersaMax Molecular Devices, USA) and absorbance was measured at 475 nm. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. ,, [28]. To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. S. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. Four wash/rinse bottles are included and configurable for both 96- and 384. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. That's why our team is at your disposal the majority of time. 2. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Concluding Remarks. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. Molecular Devices Corporation. ¨ We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. . SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. Table of Contents. The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. (VersaMax, Molecular Devices, USA). All samples were measured in triplicate. Ensure data accuracy of your reader. This manual is subject to change without notice. S. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. Multichannel optics. 747. HT22 cells are derived from hippocampal neuronal HT4 cells. Spectranet customer portalThe absorbance was recorded at 532 nm using a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, USA). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. Molecular Devices GMBH Tel. $1,749. Printed in U. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. $1,195. Cell viability was expressed as percent survival of untreated cells. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. ¨Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. The. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. Oil red O staining. After freeze-drying, the. 9000-0756. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). Table of Contents. harveyi BB152 was used as the AI-2-producing positive control (prepared as reported (11)). 47 shipping. 635. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. Accession codes. StakMax Microplate Handling System. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Products : General, Microplate Readers. SpectraMax® Microplate Reader User Manual Download Page. It was verified that absorbance values were proportional to the amount of a bound peroxidase-labeled antibody. Statistical Analysis. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Concluding Remarks. Sale Sold out. SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. (Molecular Devices, Sunnyvale, CA). # SMP54-GXP- 10UL) Pyrochrome Assay. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. Get pricing and purchase lab equipment and instrumentation today. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. 23,225, Thermo Fisher Scientific). 00 USD. Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). New and used Molecular Devices VersaMax's for sale on LabX. SpectraMax® i3 Multi-Mode Detection Platform. We use. Figure S2: The cellular localization of NDRG1 is not affected by N- or C- terminal deleted NDRG1 mutants under hypoxic conditions. After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. We would like to show you a description here but the site won’t allow us. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Ann Arbor, MI, USA). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 99 + $42. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The optical densities (ODs) of the reactants were measured at 450 nm using a microplate spectrophotometer (Versamax, Molecular Devices, CA, USA). Published Dec 16, 2020 | Updated May 19, 2023. A. . It features a modular design that allows users to add or change detection cartridges according to their needs. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The emodin thermoreversible PG was. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). (JC-1) (Molecular Probes), respectively. 5°C @ 37 °C W. OptiMax (Versamax non EXT) B0: Screenstation: all: Skanstacker: all: Skanwasher 300: all: Skanwasher 400: all: SpectraMax 190: N0:. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Finally, the absorbance at 450 nm was detected by an ELISA reader, VERSAmax (Molecular Devices, San Jose, CA, USA). Used. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. 8. Versamax Microplate Reader dibangun di sekitar monokromator kisi. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). Search for used versamax. Add to Cart. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. TECHNICAL NOTE. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. Use Windows Search to search for “Add or Remove”. Louis, USA) was used to. The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). , St. The Versamax Microplate Reader is built around a grating monochromator. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. Designed to provide the simplicity, flexibility and power required for advanced data analysis. For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). All experiments were repeated three times. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Indocyanine Green Depuration Assay. 00 Sale price $ 0. The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. New and used Molecular Devices VersaMax's for sale on LabX. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. 1. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 4. Direct cytotoxicity of HVJ-E and Laserphyrin®-HVJ-EThe MTT formazan crystals were dissolved by adding 50 μl DMSO to each well and the plates gently shaken until the MTT solution was dissolved. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. 0A-Sunnyvale, CA-Made in USASpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Each sample has a discrete sample beam and reference beam so that each well is measured directly. 3, Molecular Devices). Lihat Video. 99 shipping. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. 0A Sunnyvale, CA Made in USA VersaMax price. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). 4. g. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. P. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). 0. 17. 3860 N First Street San Jose,CA 95134 USA +1 408. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. $199. , Sunnyvale, CA, USA). Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 0 software (GraphPad). 003 OD ±1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Find regional support. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Download PDF versions of the SpectraMax Microplate Reader User Guides. Cell proliferation assay MDA-MB-231 cells were seeded at a density. Find Fanuc, Molecular Devices, Eagle, and GE for sale on Machinio. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. Molecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). Full size table. The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. Using a Spectradrop Micro-Volume Microplate (VersaMax, Molecular Devices, CA, USA), RNA was quantified, and 1 μg of total RNA was treated with DNase I (Thermo Scientific, Waltham, MA, USA) before. A. $199. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). Access all the resources, tools and information you need to stay productive. Molecular Devices VersaMax Microplate Reader. Turn the reader on and secure the USB cable. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 2. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. 00. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. The well plates were then washed with 1% acetic acid and dried. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. Molecular Devices SpectraMax Plus 384 Microplate Reader *Parts Only* Tecan Sunrise Microplate Reader. The collaborative space brings customers and researchers into the lab to test automated workflows for organoid culturing and screening, with guidance from in-house. , Sunnyvale, CA). 00. The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. molecular devices | 1311 orleans drive | sunnyvale, cA 94089 usA | email: info@moldev. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. Microplate Reader Shelf. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. The TBARS content was calculated according to the extinction coefficient as 155 mM −1 cm −1. The system provides automation for up to 50For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. ~ Bio-Tek ELx808 Microplate Reader $2,795. Sing a Microplate Reader (VersaMAx, Molecular Devices). Select Update Driver. p-nitrophenol (Sigma) with diluted concentrations ranging from 0 to 0. Tristar 3 Multimode Microplate Reader. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Sponsored. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). While the cell covers about. Molecular Devices memberdayakan para ilmuwan untuk memajukan diagnosis dini dan terapi yang lebih aman untuk pasien. We would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Get pricing and purchase lab equipment and. Used. The increase of absorbance at 405. S. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Proliferation was assessed by bromodeoxyuridine (BrdU) incorporation (Cell Signaling Technologies). In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. The. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. . Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. This robust model allows precise wavelength detection in. g. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. 4. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. StakMax. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. BMG Labtech - THERMOstar. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. 3 Methods 1. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. 0A Sunnyvale, CA Made in USAVersaMax price. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). This manual does not represent a commitment on the part of Molecular Devices Corporation. 0. With optimized reagents, option to operate the. After treatment, cells were collected, lysed with the lysis buffer. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. . Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. com ©2010 molecular devices, inc. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 2. Monochromator, tunable in 1-nm increments. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. Cell viabilities were presented as the ratio of the absorbance of treated cells to the absorbance of control cells cultured with an extract of HDPE. After freeze-drying, the. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. 6/10 #0120-1330d. The water extract afforded the greatest yield, and total phenolic and tannin. Model : VersaMax . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Primer design. Molecular Devices, LLC. Wavelength Selection. recombinant proteins, including monoclonal antibodies. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. 5% NP-40) supplemented with protease and phosphatase inhibitor cocktail (GenDepot, Baker, TX, USA). Cell. 0A Sunnyvale, CA Made in USA The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). Select the Driver tab. One microgram of RNA was. The absorbance of reaction mixtures was read at 475 nm using a microplate reader (VERSAMax, Molecular Devices) to determine the quantity of dopachrome produced. Spectranet customer portal. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). , VersaMax; Molecular Devices) Incubator for cell culture. Manufacturer: Molecular Devices The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices Corporation. S. 0200-6117. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. A. MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Used. Microplate Reader Capability > UV - Vis Absorbance. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Finally, the absorbance was determined at 490 nm on a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Open the Windows Task Manager, go to the Services tab, and click Open Services. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Cellular imaging was performed on a Confocal microscope (Olympus IX 81 + FV1000, Shanghai, China) using an excitation wavelength of 380 nm. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Ensure data accuracy of your reader. 9000-0756. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. (VersaMax Molecular Devices, Sunnyvale, CA). 5) containing 0. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. VersaMax™ Tunable Microplate Reader, Molecular Devices. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. Windows Home Editions are not supported. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). In general terms, plasma was added to a 96-well plate containing the targeted antisera, after rigorous washing steps and conjugation with a secondary detection antisera, and the resultant colorimetric reaction was read by scanning spectrophotometry (VersaMax, Molecular Devices, Sunnyvale, CA). . Get pricing and purchase lab equipment and. , Sunnyvale, CA). Monday to Friday 8am - 5pm GMT. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. SoftMax Pro. Then, the cultured cells were treated with different. The sections were stained with hematoxylin and eosin (H&E) and. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995.